Two‐dimensional crystals of the Kdp‐ATPase of Escherichia coli
- 4 November 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 396 (2-3) , 172-176
- https://doi.org/10.1016/0014-5793(96)01096-4
Abstract
A variant form of the Kdp-ATPase of Escherichia coli was overproduced to a level approaching 37% of the protein in the inner membrane of this organism. Membranes from overproducing cells were prepared with an inside-out orientation. Incubation of the membranes on ice for 1-2 weeks in the presence of sodium vanadate resulted in the formation of two-dimensional crystals of the Kdp-ATPase. The calculated projection map of the p1 crystal form showed three prominent density peaks at a resolution of 22 A. This technique is a useful and simple method to obtain low-resolution structures of membrane proteins.Keywords
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