Bacteriocin typing by leakage of ultraviolet light-absorbing material

Publisher Website
Google Scholar
Abstract
A rapid and reproducible method of bacteriocin typing is described based on leakage of UV light-absorbing material (UVAM) detectable in supernatants of bacteriocin-sensitive cultures by a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard error of the mean, are a set of standardized bacteriocins, produced under defined conditions and of determined strength. These must interact with the unknown bacterial culture in suspension and at a given ratio in order to achieve an optimal multiplicity of interaction. Pyocin and colicin typing by the scrape and streak technique of Gillies was compared with the UVAM leakage method in 275 tests; the 2 tests were in good agreement for the strains tested. [Pseudomonas aeruginosa, Escherichia coli and Shigella sonnei were studied.].

This publication has 33 references indexed in Scilit: