Distribution of a negative regulator of haematopoietic stem cell proliferation (AcSDKP) and thymosin, β4 in mouse tissues
- 9 September 1991
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 289 (2) , 171-175
- https://doi.org/10.1016/0014-5793(91)81062-d
Abstract
A competitive enzyme immunoassay using acetylcholinesterase as tracer for thymosin β4, has been developed. Using this assay and a previously described EIA for AcSDKP, a negative regulator of pluripotent haematopoietic stem cell proliferation, the levels of these two peptides were determined in mouse tissue extracts. The combination of EIAs with different HPLC procedures validated these methods and clearly demonstrated the ubiquity of these peptides in mouse tissues. Similar results are reported for rabbit thymus which suggest different hypotheses for AcSDKP biosynthesis.Keywords
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