Presence of Plasma Membrane-bound Ca2+-ATPase in the Secretory Epithelia of the Inner Ear

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Abstract
The Ca2+ concentration of the endolymph is low, around 0.023 mM. Yet, because of the positive endocohlear potential, Ca2+ must be actively transported into the endolymphatic space. The mechanisms responsible for the active Ca2+ transport into the endolymph are not known. In this study, the presence of plasma membrane-bound Ca2+-ATPase (PMCA ATPase) in the endolymph-producing, secretory epithelia of the inner ear from guinea pig was investigated with immunoblotting and immunohistochemistry. The antibody used was a monoclonal antibody which recognizes an epitope shared by all four known isoforms of PMCA ATPase. With immunoblotting, a band corresponding to PMCA ATPase was found in the stria vascularis, the ampullary tissue, the utricle and the endolymphatic sac in assays from at least three different batches of tissue. With immunohistochemistry, a strong positive staining reaction for PMCA ATPase could be seen in the stria vascularis and the dark cells of the ampullary tissue and the utricle. The epithelial cells in the endolymphatic sac showed a moderate positive staining reaction. Accordingly, in this study the presence of PMCA ATPase was shown in all the endolymph-producing, secretory epithelia of the inner ear. These results indicate that PMCA ATPase plays a role in the regulation of the Ca2+ concentration in the endolymph.