A method for enucleating oocytes ofXenopus laevis
Open Access
- 1 February 1977
- journal article
- research article
- Published by The Company of Biologists in Development
- Vol. 37 (1) , 203-209
- https://doi.org/10.1242/dev.37.1.203
Abstract
A method is described for the enucleation and complete healing of Xenopus oocytes so that the enucleated oocytes withstand multiple injections and culture for several days. The oocytes are defolliculated and enucleated manually and allowed to heal in a potassium phosphate buffer. Oocytes enucleated in this way support RNA synthesis by injected HeLa-cell nuclei 3 days later. This method is valuable in providing a low-background system in which transcription of injected nuclei can be studied.This publication has 7 references indexed in Scilit:
- Injected nuclei in frog oocytes: RNA synthesis and protein exchangeDevelopment, 1976
- Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersalDevelopment, 1976
- Injected nuclei in frog oocytes provide a living cell system for the study of transcriptional controlNature, 1976
- Action of progesterone and related steroids on oocyte maturation in Xenopus laevis. An in vitro studyCell Differentiation, 1972
- Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living CellsNature, 1971
- Role of the oocyte nucleus in physiological maturation in Rana pipiensDevelopmental Biology, 1969
- ROLE OF GERMINAL VESICLE IN OOCYTE MATURATION IN ANURANS AS REVEALED BY REMOVAL AND TRANSPLANTATION OF NUCLEI1964