GIRK1 immunoreactivity is present predominantly in dendrites, dendritic spines, and somata in the CA1 region of the hippocampus
- 4 February 1997
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (3) , 1007-1012
- https://doi.org/10.1073/pnas.94.3.1007
Abstract
Electron microscopic analysis of the CA1 region of the rat hippocampus revealed that specific immunoreactivity (IR) for a G protein-gated, inwardly rectifying potassium channel (GIRK1) was present exclusively in neurons and predominantly located in spiny dendrites of pyramidal cells. Within stratum lacunosum-moleculare and the superficial stratum radiatum, GIRK1-IR was often present immediately adjacent to asymmetric (excitatory-type) postsynaptic densities in dendritic spines. The subcellular localization of GIRK1-IR in the Golgi apparatus of pyramidal cell somata and in the plasma membrane of dendrites and dendritic spines confirms the hypothesis that GIRK1 is synthesized by pyramidal cells and transported to the more distal dendritic processes. G protein-coupled receptor activation of a dendritic potassium conductance would attenuate the propagation of excitatory synaptic inputs and thereby produce postsynaptic inhibition. Thus, these results show that the GIRK family of channels joins the list of voltage-sensitive channels now known to be expressed in dendritic spines.Keywords
This publication has 42 references indexed in Scilit:
- Dendritic Integration in Mammalian Neurons, a Century after CajalPublished by Elsevier ,1996
- Inhibition of an inwardly rectifying K+ channel by G-protein α-subunitsNature, 1996
- Evidence that direct binding of Gβγ to the GIRK1 G protein-gated inwardly rectifying K+ channel is important for channel activationNeuron, 1995
- Heterologous Multimeric Assembly Is Essential for K+ Channel Activity of Neuronal and Cardiac G-Protein-Activated Inward RectifiersBiochemical and Biophysical Research Communications, 1995
- Cloning provides evidence for a family of inward rectifier and G‐protein coupled K+ channels in the brainFEBS Letters, 1994
- A region of the muscarinic-gated atrial K+ channel critical for activation by G protein βγ subunitsNeuron, 1994
- Activation of the cloned muscarinic potassium channel by G protein βγ subunitsNature, 1994
- Dendritic spines: role of active membrane in modulating synaptic efficacyBrain Research, 1985
- Comparison of fixation and penetration enhancement techniques for use in ultrastructural immunocytochemistry.Journal of Histochemistry & Cytochemistry, 1983
- Adenosine depression of hippocampal neurons in vitro: An intracellular study of dose-dependent actions on synaptic and membrane potentialsNeuroscience Letters, 1981