Evidence for an interaction between cytosolic aldolase and the ATP‐ and pyrophosphate‐dependent phosphofructokinases in carrot storage roots
- 30 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (3) , 277-280
- https://doi.org/10.1016/0014-5793(92)81208-4
Abstract
Immunoaffinity chromatography was employed to identify potential plant cytosolic aldolase (ALDc) binding proteins. A clarified homogenate of carrot storage root was chromatographed on a column of protein‐A—Sepharose that had been covalently coupled to anti‐(carrot root ALD,) immunoglobulin G. The column was washed with phosphate‐buffered saline (PBS), followed by step‐wise elution with increasing concentrations of NaCl in PBS. Several proteins were eluted following application of the salt gradient. Western blotting identified the major eluting proteins to be the PPi‐dependent phosphofructokinase (PFP) and the cytosolic form of the ATP‐dependent phosphofructokinase (PFKc), enzymes that are metabolically sequential to ALDc. The results suggest that ALDc may specifically interact with PFP and PFKc in carrots.Keywords
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