Alkalimonas collagenimarina sp. nov., a psychrotolerant, obligate alkaliphile isolated from deep-sea sediment

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Abstract
A psychrotolerant, obligately alkaliphilic, collagenolytic enzyme-producing bacterium, strain AC40T, was isolated from a deep-sea sediment off Torishima Island, Japan, at a depth of 4026 m. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genusAlkalimonas, with highest sequence similarity (97.9 %) toAlkalimonas delamerensis1E1T. DNA–DNA hybridization experiments of strain AC40TwithA. delamerensis1E1Trevealed a level of relatedness of less than 30 %. Cells of strain AC40Twere strictly aerobic, rod-shaped, Gram-negative and motile by means of a single polar flagellum. The organism grew over a range of temperatures from 5 to 37 °C and at initial pH values between 7.0 and 10.5. Optimal growth was observed at 33 °C and at pH 8.5–10.0. Cellular fatty acids of strain AC40Twere predominantly saturated and mono-unsaturated straight-chain components (C16 : 0and C18 : 1). The major isoprenoid quinone was Q-8. The G+C content of the DNA was 49.3 mol%. Phylogenetic characteristics, physiological properties and DNA–DNA hybridization data indicate that strain AC40Trepresents a novel species of the genusAlkalimonas, for which the nameAlkalimonas collagenimarinasp. nov. is proposed. The type strain is AC40T(=JCM 14267T=NCIMB 14266T).

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