DNA binding specificity and sequence of Xanthomonas campestris catabolite gene activator protein-like protein
- 1 August 1992
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (16) , 5457-5461
- https://doi.org/10.1128/jb.174.16.5457-5461.1992
Abstract
The Xanthomonas campestris catabolite gene activator protein-like protein (CLP) can substitute for the Escherichia coli catabolite gene activator protein (CAP) in transcription activation at the lac promoter (V. de Crecy-Lagard, P. Glaser, P. Lejeune, O. Sismeiro, C. Barber, M. Daniels, and A. Danchin, J. Bacteriol. 172:5877-5883, 1990). We show that CLP has the same DNA binding specificity as CAP at positions 5, 6, and 7 of the DNA half site. In addition, we show that the amino acids at positions 1 and 2 of the recognition helix of CLP are identical to the amino acids at positions 1 and 2 of the recognition helix of CAP:i.e., Arg at position 1 and Glu at position 2.Keywords
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