An Abrasive Paper Technique for Preparing Sections of Wood for Microscopic Study
- 1 January 1958
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 33 (3) , 103-108
- https://doi.org/10.3109/10520295809111832
Abstract
Dry wood specimens are sawed to 2mm thickness and impregnated with resin such as Lewisol 28 (Hercules Powder Co.). One side of the specimen is ground by hand on abrasive papers of grades #100, #180, #240, and #320. This side is then cemented to a petrographic glass slide with stick shellac and the other side similarly ground. Scratches can be eliminated by scraping the ground surface with the sharp edge of an ordinary glass microscopic slide. The section is removed by heating the slide, dissolving the shellac with alcohol and the resin of the embedding matrix with xylene. The sections can be stained in a hot saturated alcoholic solution of safranin O, counterstained with 0.01% fast green in an equal parts mixture of clove oil, methyl cellosolve, and absolute alcohol, and mounted in balsam.Keywords
This publication has 1 reference indexed in Scilit:
- Cutting Microscopic Sections of Wood Without Previous Treatment in Hydrofluoric AcidStain Technology, 1930