A Novel Expression Vector for the Cyanobacterium, Synechococcus PCC 6301

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Abstract
A cyanobacterial expression vector was constructed using ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) promoter and terminator sequences derived from Synechococcus PCC 6301. The recombinant plasmid, designated pARUB19, has an ampicillin-resistant (Ap R ) gene as a selectable marker and four unique restriction sites to allow the insertion of foreign genes. Using this vector, the luciferase gene from the firefly, Photinus pyralis , was introduced into Synechococcus PCC 6301 cells. The luciferase expression vector could be maintained stably in the host cells. Light production of luciferin/luciferase was detected in the transformants. Luciferase amounted to 1.2% of the total soluble protein. This plasmid may facilitate higher levels of foreign gene expression in Synechococcus PCC 6301.

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