THE BINDING OF PYROPHOSPHATE TO MYOSIN A AND MYOSIN B

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Abstract
The number of molecules of ATP (adenosine triphosphate) and PP (pyrophosphate) found is determined by the change in light scattering intensity or by the equilibrium-dialysis method. Myosin B binds 1 molecule ATP or PP/unit weight (4.6 x 105 g) while myosin A is combined with 2 molecules PP/unit weight (2.3 x 105 g). The change in light scattering intensity approximately parallels the extent of PP binding to myosin B. Myosin A possesses 2 binding sites: the one is linked to actin to form myosin B and another for binding with ATP or PP.