Somatic Variation in Resistance of Populus to Septoria musiva

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Abstract
Tissue culture of hybrid poplars previously susceptible to leaf spot caused by Septoria musiva was used to obtain poplars with putative resistance. Stem internode explants were used to obtain proliferating callus cultures on which adventitious bud formation and shoot proliferation were induced. Elongated shoots were excised and rooted in a peat-perlite medium under high humidity and transferred to the greenhouse. Variant plants were selected among the regenerants using a leaf disk bioassay that distinguished resistant plants. The incidence of somaclonal variation differed among genotypes. Tissue culture of poplars is potentially useful in detecting and recovering somaclonal variation in resistance to Septoria.

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