IMMUNOCHEMICAL STUDIES OF ADRENOCORTICOTROPIN USING TRITIUM LABELED HORMONE
- 1 May 1980
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 15 (5) , 480-484
- https://doi.org/10.1111/j.1399-3011.1980.tb02925.x
Abstract
A radioimmunoassay using specifically tritiated corticotropin (3H-ACTH) of high specific radioactivity (46 Ci/mmol) and full biological activity has been developed. The assay is sensitive to ACTH in the range 200 pg-10 ng. 50% displacement of 3H-ACTH is produced by less than a twofold excess of unlabeled hormone. Immunochemical studies with various ACTH analogs showed that the antiserum recognized the intact molecule better than any of the fragments.Keywords
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