Inability to demonstrate calcite precipitation by bacterial isolates from travertine

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Abstract
Bacteria were isolated from 10 actively depositing travertine sites in Europe and North America. Forty‐four isolates were characterized and 33 (75%) were identified as Pseudomonas. Thirty isolates produced base on glucose‐peptone media and 32 isolates produced ammonia from amino acids. Because of the low bacterial biomass in the travertines, the formation of bases, including ammonia was not considered to be significant in carbonate precipitation. None of the bacteria was observed to precipitate calcite from supersaturated calcium carbonate solutions in vitro.