THE INFLUENCE OF CORTISONE AND INSULIN ON THE RESPIRATORY ACTIVITY OF RAT ADIPOSE TISSUE IN VITRO1

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Abstract
O2 consumption and RQ of brown and white adipose tissue of 24-hour fasted normal and cortisone-treated rats were measured in vitro with and without glucose and insulin. O2 consumption of brown fat was significantly higher than that of white adipose tissue and was not modified by any experimental condition studied. The RQ of white adipose tissue was significantly higher than that of brown adipose tissue and was increased by incubation with glucose, but not modified by insulin. In various experiments pretreatment with cortisone for 48 hours either induced no change or a decrease in the RQ of adipose tissue incubated in medium alone, in glucose medium and in glucose and insulin medium. With white adipose tissue the increase in RQ with glucose was prevented by cortisone pretreatment. These results are consistent with the interpretation that cortisone inhibits lipogenesis from carbohydrate but offer no basis to interpret the previously reported effects of insulin and cortisone on adipose tissue glycogen levels.