Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes
- 10 February 1983
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 301 (5900) , 482-488
- https://doi.org/10.1038/301482a0
Abstract
Purified RNA polymerase II from calf thymus can bind to about 15% of the nucleosome core particles prepared from mouse myeloma cells, forming a discrete complex having a sedimentation coefficient of 18S. These bound nucleosome cores are heavily enriched in transcribed DNA sequences, are deficient in histones H2A and H2B, and undergo a reversible change in structure on RNA polymerase II binding.Keywords
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