K-582, a new peptide antibiotic. I.

Abstract
A new basic peptide antibiotic designated as K582 was isolated, purified and characterized. When K582 was applied to a column of Al2O3 or Bio-Gel P-2 or CM Sephadex, 2 major peaks which were named Fraction I (K 582A) and Fraction II (K 582B) were obtained. The N content, the behavior in color reaction, the absorption bands of amide linkages in the IR absorption spectrum, 1H NMR spectrum and 13C NMR spectrum indicated the peptide nature of K 582A and K 582 B. K 582 was effective against yeasts, but inactive against other gram-positive bacteria, gram-negative bacteria and Mycobacterium. The toxicity was low in mice.

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