Quantification of RT-PCR Products: Ethidium Bromide—Stained Gel Analysis Compared With Fluorescent Detection Using an Automated Sequencer
- 1 June 1999
- journal article
- Published by Oxford University Press (OUP) in Laboratory Medicine
- Vol. 30 (6) , 419-422
- https://doi.org/10.1093/labmed/30.6.419
Abstract
Reverse transcription–polymerase chain reaction (RT-PCR) is a powerful tool to study gene expression and is used increasingly in clinical medicine and predictive oncology. We compared two techniques for semiquantitation of interleukin-6 RT-PCR products: video image analysis of ethidium bromide-stained gel and a fluorescent-based technique using an automated sequencer. We found a significant correlation between the two detection techniques (P < .01) in our series of 64 samples. Our results strongly suggest good reliability of the video image analysis compared with sequencer quantitation; video image analysis could be a good alternative for laboratories without a sequencer.Keywords
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