Fermentation to Short-Chain Fatty Acids and Lactate in Human Faecal Batch Cultures Intra- and Inter-Individual Variations versus Variations Caused by Changes in Fermented Saccharides
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Gastroenterology
- Vol. 26 (12) , 1285-1294
- https://doi.org/10.3109/00365529108998626
Abstract
The fermentation to short-chain fatty acids, lactate, and ammonia from several non-starch polysaccharides, glucose, and albumin was investigated in 16.6% faecal homogenates. Increasing concentrations (0-30 mg/ml) of glucose, wheat bran, pectin, ispaghula, cellulose, or albumin incubated for 24 h in homogenates pooled from three individuals increased short-chain fatty acid production linearly. Amounts and ratios of short-chain fatty acids formed were highly dependent on the type of substrate fermented. Fermentable saccharides increased ammonia assimilation, in contrast to the metabolic inert cellulose. Nine faecal homogenates sampled from three individuals at three occasions were incubated for 6 and 24 h. The production of total short-chain fatty acids, acetate, propionate, and butyrate and the accumulation of D- and L-lactate changed considerably in relation to the type of substrate added (cellulose, ispaghula, wheat bran, pectin, gum arabic, and glucose; p < 10−4-10−7). In contrast, there were no significant (p > 0.05) differences in organic acid formation between the nine homogenates, and the intra- and inter-individual variations were of the same magnitude. Variations in fermentation, when measured as organic acid formation, were therefore related to the type of substrate fermented rather than the individual tested.Keywords
This publication has 29 references indexed in Scilit:
- Constancy of glucose and starch fermentations by two different human faecal microbial communities.Gut, 1989
- Effect of Human Faecal Donor on in Vitro Fermentation VariablesScandinavian Journal of Gastroenterology, 1989
- Degradation of Amino Acids to Short-Chain Fatty Acids in Humans: An in Vitro StudyScandinavian Journal of Gastroenterology, 1988
- Super-efficient starch absorptionDigestive Diseases and Sciences, 1987
- The Influence of Ispaghula Husk and Lactulose on the In Viva and the In Vitro Production Capacity of Short-Chain Fatty Acids in HumansScandinavian Journal of Gastroenterology, 1987
- Carbohydrate FermentationPublished by Elsevier ,1983
- Carbohydrate Metabolism in the Human ColonPublished by Elsevier ,1983
- Incomplete Absorption of the Carbohydrate in All-Purpose Wheat FlourNew England Journal of Medicine, 1981
- COLONIC RESPONSE TO DIETARY FIBRE FROM CARROT, CABBAGE, APPLE, BRAN, AND GUAR GUMThe Lancet, 1978
- Pretreatment methods prior to gaschromatographic analysis of volatile fatty acids from faecal samplesClinica Chimica Acta; International Journal of Clinical Chemistry, 1977