Investigations on the Growth and Metabolism of Plant Cells

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Abstract
This paper describes the technique of growing small discs of carrot phloem in pure culture and with the precautions necessary to standardize the rate of growth at the highest practicable level. It deals with the procedure necessary to remove replicate samples of the material, with variability along the radial and longitudinal axes of the carrot, and with the regulation of the culture conditions. Evidence is adduced of the existence of a ‘staling’ factor in crowed cultures.