Inactivation of Microbiological Activity of Crystalline Vitamin B12 by Reducing Agents.

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Abstract
The addition of a reducing agent such as ascorbic acid, cysteine HC1, thiamine or hydroquinone to a soln. of vit. B12 resulted in the loss of microbiologic activity. The rate of inactivation was dependent on the hydrogen-ion concn. and on the reducing power of the inactivating agents. The destruction of the microbiologic activity was not accompanied by a similar lowering of potency of the vit. B12 as animal protein factor.
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