Survival of Fungus Cultures Maintained under Mineral Oil for Twelve Years

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Abstract
Cultures of fungi and Actino-mycetes comprising 181 human pathogens and 21 "saprophytes" were transferred to Sabouraud dextrose agar slants, incubated at room temperature for 2 wk, covered with heavy mineral oil and stored at room temperature. Of the 202 cultures stored, 171 were viable after 6 yr. and 151 and 12 yrs. Prominent among the species of which most strains failed to survive 6 yrs. were Sporothrix schenckii and saprophytic species of Cryptococcus; in addition were Fonsecaea pedrosoi, Paracoccidioides brasiliensis and Nocardia asteroides at 12 yrs. The pattern of viability at the end of 12 yrs. was usually similar qualitatively to that after 6 yrs., the decrease in the number of viable strains occurring chiefly among the same species affected earlier.

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