CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.
- 15 June 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (12) , 5549-5553
- https://doi.org/10.1073/pnas.90.12.5549
Abstract
PCR was used to clone isolates of the human immunodeficiency virus type 1 (HIV-1) nef gene directly from peripheral blood leukocytes of HIV-1-infected individuals. A transient expression system with human CEM T cells was used to assess the effect of nef on CD4 antigen expression on the cell surface. We show that CD4 down-regulation is a frequent property of primary HIV-1 nef alleles. Mutations in conserved amino acid motifs of Nef disrupted CD4 down-regulation. Our observations strongly suggest that CD4 down-regulation reflects a conserved function of nef, which is selected in vivo in human HIV-1 infection. Methodology described here provides quantitative assays to establish whether alterations in nef correlate with the dynamics of disease progression in human AIDS.Keywords
This publication has 22 references indexed in Scilit:
- Protective Effects of a Live Attenuated SIV Vaccine with a Deletion in the nef GeneScience, 1992
- Activation Pathways and Human Immunodeficiency Virus Type 1 Replication Are Not Altered in CD4+T Cells Expressing thenefProteinAIDS Research and Human Retroviruses, 1992
- Importance of the nef gene for maintenance of high virus loads and for development of AIDSCell, 1991
- Serine phosphorylation-independent downregulation of cell-surface CD4 by nefNature, 1991
- Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolationsCell, 1989
- Nef Protein of HIV-1 Is a Transcriptional Repressor of HIV-1 LTRScience, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene productNature, 1987
- Cytoplasmic localization of the HTLV-III 3′orf protein in cultured T cellsVirology, 1986
- Dideoxy sequencing method using denatured plasmid templatesAnalytical Biochemistry, 1986