Compilation and analysis of 54-dependent promoter sequences
- 1 November 1999
- journal article
- review article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 27 (22) , 4305-4313
- https://doi.org/10.1093/nar/27.22.4305
Abstract
Promoters recognized by the RNA-polymerase with the alternative sigma factor sigma(54) (E sigma(54)) are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site, instead of the typical -35 and -10 boxes. Here we compile 186 -24/-12 promoter sequences reported in the literature and generate an updated and extended consensus sequence. The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters. The effect of several reported mutations at the -24/-12 elements on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus.This publication has 141 references indexed in Scilit:
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