Determination of Proteins with Fast Red VR by a Corrected Resonance Light-Scattering Technique
- 28 February 2003
- journal article
- Published by Springer Nature in Analytical Sciences
- Vol. 19 (2) , 211-215
- https://doi.org/10.2116/analsci.19.211
Abstract
A simple corrected resonance light-scattering (CRLS) technique was established to correct for any distortion of the resonance light scattering (RLS) spectra resulting from molecular absorption. By using an absorption cell holder to change the propagation direction of the incident light beam of a common spectrofluorometer, the molecular absorption was directly measured through a spectrofluorometer. With measurements of the CRLS signals of the interaction of Fast Red VR (FRV) and proteins, we proved that the present correction for the RLS spectra in terms of the molecular absorption of excitation and scattering radiation can improve the detection sensitivity by about two fold.Keywords
This publication has 22 references indexed in Scilit:
- Determination of proteins with α,β,γ,δ-tetrakis(4-sulfophenyl)porphine by measuring the enhanced resonance light scattering at the air/liquid interfaceAnalytica Chimica Acta, 2001
- Separation of Scattering and Absorption Contributions in UV/Visible Spectra of Resonant SystemsAnalytical Chemistry, 2001
- Determination of chlortetracycline in body fluids with the complex cation of chlortetracycline–europium(III)–trioctylphosphine oxide by total internal reflected fluorescence at a water/tetrachloromethane interfaceAnalytica Chimica Acta, 2001
- Sensitive and simple determination of protein by resonance Rayleigh scattering with 4-azochromotropic acid phenylfluoroneAnalytica Chimica Acta, 2000
- Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Alcian Blue 8GX.Analytical Sciences, 2000
- Study on the interaction of protein with Sulfonazo III by Rayleigh light scattering technique and its applicationAnalytica Chimica Acta, 1999
- Resonance Light Scattering and Its Application in Determining the Size, Shape, and Aggregation Number for Supramolecular Assemblies of ChromophoresThe Journal of Physical Chemistry B, 1999
- Resonance light scattering study of aggregation of two water soluble porphyrins due to their interaction with bovine serum albuminAnalytica Chimica Acta, 1997
- Microdetermination of proteins by Rayleigh light scattering technique with acid green 25Analytica Chimica Acta, 1997
- Determination of Nanograms of Nucleic Acids by Their Enhancement Effect on the Resonance Light Scattering of the Cobalt(II)/4-[(5-Chloro-2-pyridyl)azo]-1,3-diaminobenzene ComplexAnalytical Chemistry, 1997