Cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo
- 1 July 1987
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 13 (4) , 325-333
- https://doi.org/10.1007/bf01534926
Abstract
As part of our mouse model of somatic mutation, we have begun to characterize spontaneously occurring hypoxanthine phosphoribosyltransferase (HPRT) — deficient mouse lymphocytes. Lymphocytes were cloned by in vitro exposure of spleen cells from male C57B1/6 mice to the mitogen concanavalin A, conditioned medium containing lymphocyte growth factors, and thioguanine (TG), in a limiting dilution assay. The 17 TG-resistant clones recovered were all highly deficient in HPRT activity and were found by analysis of surface antigens to be representative of the major subclasses of T lymphocytes. Southern analysis of lymphocyte genomic DNA detected alterations of the hprtgene in 12/17 of the HPRT-deficient lymphocyte clones. Of these 12, 2/17 were lacking the entire hprtlocus, 7/17 lacked part of the locus, and 3/17 had other, unidentified alterations.This publication has 48 references indexed in Scilit:
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