Immunochemical Studies on Proteins Important in Blood Coagulation

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Abstract
This report draws attention to the fact that only minute quantities of purified serum proteins (1 —15 μg/kg body weight) are required to stimulate antibody production. Injection of amounts greater than 1 mg/kg either is a waste or favors the production of non-specific antisera. Potent antisera can be produced in rabbits with a single intramuscular injection using either Al(OH)3 gel or Amphojel as an adjuvant. A number of purified plasma protein preparations which appear homogenous by ultra centrifugation or biological activity studies actually contain several antigenic components and are immunologically heterogeneous, when checked with highly specific antisera. Fibrinogen prepared by alcohol fractionation and tannic acid purification was found to be free of prothrombin, antihemophilic factor and Profibrinolysin. Both Immunoelectrophoresis and double diffusion in agar indicated a homogeneous preparation. Fibrinogens prepared from human, canine and bovine plasmas exhibited a low species specificity. * Aided by a research grant from the Michigan Heart Association and research grant H-4712 from the National Heart Institute, National Institutes of Health, United States Public Health Service. ** Present address Ortho Research Foundation, Raritan, N. J.