Resistance of Tomato and Pepper to T3 Strains of Xanthomonas campestris pv. Vesicatoria Is Specified by a Plant-Inducible Avirulence Gene
Open Access
- 1 September 2000
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 13 (9) , 911-921
- https://doi.org/10.1094/mpmi.2000.13.9.911
Abstract
Tomato race 3 (T3) of Xanthomonas campestris pv. vesicatoria (Xcv) elicits a hypersensitive response (HR) in leaves of Lycopersicon esculentum near-isogenic line (NIL) 216 and pepper genotypes. One cosmid clone (35 kb) selected from a genomic library of a T3 strain induced an HR in all resistant plants. A 1.5-kb active subclone containing the putative avirulence (avr) gene, designated avrXv3, was sequenced. The avrXv3 gene encodes a 654-bp open reading frame (ORF) with no homology to any known gene. Expression studies with a fusion of this gene and uidA indicated that avrXv3 is plant inducible and controlled by the hypersensitivity and pathogenicity (hrp) regulatory system. Mutational analysis and transcription activation assays revealed that AvrXv3 has transcription activation activity in yeast, and that the putative domain responsible for that activity is located at the C terminus of the AvrXv3 protein. Agrobacterium tumefaciens-mediated transient expression confirmed the direct role of AvrXv3 in elicit...Keywords
This publication has 54 references indexed in Scilit:
- Five Avirulence Genes from Xanthomonas campestris pv. malvacearum Cause Genotype-Specific Cell Death When Expressed Transiently in CottonMolecular Plant-Microbe Interactions®, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Transcriptional activation by recruitmentNature, 1997
- Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto KinaseScience, 1996
- HrpG, a KeyhrpRegulatory Protein ofXanthomonas campestrispv.vesicatoriaIs Homologous to Two-Component Response RegulatorsMolecular Plant-Microbe Interactions®, 1996
- Sequence and Expression Analysis of thehrpBPathogenicity Operon ofXanthomonas campestrispv.vesicatoriaWhich Encodes Eight Proteins with Similarity to Components of the Hrp, Ysc, Spa, and Fli Secretion SystemsMolecular Plant-Microbe Interactions®, 1995
- Different Phenotypes Associated with Incompatible Races and Resistance Genes in Bacterial Spot Disease of PepperPlant Disease, 1987
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980
- Current Status of the Gene-For-Gene ConceptAnnual Review of Phytopathology, 1971
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969