Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants
- 1 May 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 130 (2) , 746-749
- https://doi.org/10.1128/jb.130.2.746-749.1977
Abstract
Yeast strains carrying recessive mutations representing 4 different loci that cause defects in pyruvate kinase [EC 2.7.1.40], pyruvate decarboxylase [EC 4.1.1.1], 3-phosphoglycerate kinase [EC 2.7.2.3] and 3-phosphoglycerate mutase [EC 5.4.2.1] were isolated and partially characterized. Cells carrying these mutations were unable to use glucose as a C source as measured in turbidimetric growth experiments. Tetrad analysis indicated that these mutations were not linked to each other; 1 of the mutations, that affecting phosphoglycerate kinase, was located on chromosome III.This publication has 17 references indexed in Scilit:
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