ISOLATION OF PURIFIED H AND L POLYPEPTIDE CHAINS FROM GUINEA-PIG GAMMA2-IMMUNOGLOBULIN AFTER MILD REDUCTION

  • 1 January 1966
    • journal article
    • research article
    • Vol. 10  (4) , 309-+
Abstract
A method is described whereby biologically active H and L chains, free from each other by immunochemical criteria, can be prepared from guinea pig [gamma] 2-immunoglobulin. The procedure makes use of mile reduction and alkylation in the absence of a denaturing agent, followed by gel filtration in the presence of 4 M guanidine.HCl. Specific binding of antigen by the method of equilibrium dialysis was found only when specific H chains were mixed with L chains, with more activity resulting when the L chains were also specific. Specific binding by antibody H chains alone was not found.