Identification and Discrimination of Burkholderia pseudomallei , B. mallei , and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes
Open Access
- 1 December 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (12) , 5892-5894
- https://doi.org/10.1128/jcm.42.12.5871-5874.2004
Abstract
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis , a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.Keywords
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