Bleomycin resistance conferred by a drug‐binding protein
- 28 March 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 230 (1-2) , 171-175
- https://doi.org/10.1016/0014-5793(88)80665-3
Abstract
The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E.coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein.Keywords
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