Peptidyl puromycin synthesis; effect of several antibiotics which act on 50 S ribosomal subunits
- 12 February 1971
- journal article
- Published by Wiley in FEBS Letters
- Vol. 13 (1) , 65-67
- https://doi.org/10.1016/0014-5793(71)80666-x
Abstract
The effects of several antibiotics which are know to bind with 50 S ribosomal subunits, on the formation of several di- and tri-peptidyl puromycins have been examined. Tylosin and spiramycin inhibited the formation of phenylalanyl-14C-phenylalanyl-puromycin, glycyl-14C-phenyllalanyl-puromycin, leucyl-14C-phenylalanyl-puromycin, N ϵ-carbobenzoxylysyl-14C-phenylalanyl-puromycin, and valyl-glycyl-14C-phenylalanyl-puromycin as well as N-acetyl-14C-phenylalanyl-puromycin. Of these compounds, erythromycin and oleandomycin selectively inhibited the formation of phenylalanyl-14C-phenylalanyl-puromycin. Although chloramphenicol and lincomycin inhibited the formation of most of these peptidyl puromycins, the formation of phenylalanyl-14C-phenylalanyl-puromycin and leucyl-14C-phenylalanyl-puromycin was found to be resistant to these antibiotics. So far, no significant effect of siomycin has been observed on pepetidyl puromycin formation in the absence of G factor.Keywords
This publication has 20 references indexed in Scilit:
- Reaction of Puromycin with Chemically Prepared Peptidyl Transfer RNAEuropean Journal of Biochemistry, 1970
- Effect of siomycin on protein synthesizing activity of Escherichia coli ribosomesBiochemical and Biophysical Research Communications, 1970
- Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNABiochemical and Biophysical Research Communications, 1970
- MODE OF ACTION OF SIOMYCINThe Journal of Antibiotics, 1970
- Comparative studies on the mechanism of action of lincomycin, streptomycin, and erythromycinBiochemical and Biophysical Research Communications, 1969
- STUDIES ON SIOMYCIN. VThe Journal of Antibiotics, 1969
- Mode of action of macrolidesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Ribosome-catalysed peptidyl transfer: the polyphenylalanine systemJournal of Molecular Biology, 1968
- Ribosome-catalysed peptidyl transfer: Effects of some inhibitors of protein synthesisJournal of Molecular Biology, 1967
- N-Formyl-Methionyl-sRibonucleic Acid and Chain Initiation in Protein Biosynthesis: Polypeptidyl-sribonucleic acid and amino-acyl-sribonucleic Acid Binding Sites on RibosomesNature, 1966