The Effect of in Vivo Irradiation on Macrophage Function

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Abstract
In vivo irradiation (660 R) of donor mice before removal of their peritoneal macrophages caused activation of these cells as indicated by increases in lysosomal enzymes but did not significantly affect the manner in which these cells handled keyhole limpet hemocyanin (KLH) in comparison to how macrophages from unirradiated donors handled KLH. The parameters measured included uptake, catabolism, retention and amount of membrane-bound KLH. In syngeneic recipients, however, the immunogenicity of equal amounts of KLH associated with activated macrophages from irradiated donors was significantly increased compared to that of KLH associated with macrophages from unirradiated donors. Also, addition of activated non-KLH-containing macrophages to the inoculum of normal KLH-containing macrophages increased the immune response. Infection of mice with lactic dehydrogenase virus also enhanced the immunogenicity of KLH associated with macrophages from these mice compared to control, uninfected mice.

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