Rapid and Simple Determination of the Escherichia coli Phylogenetic Group
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- 1 October 2000
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (10) , 4555-4558
- https://doi.org/10.1128/aem.66.10.4555-4558.2000
Abstract
Phylogenetic analysis has shown that Escherichia coliis composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.Keywords
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