Partial nucleotide sequence of a bovine major histocompatibility class II DRβ‐like gene

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Abstract
A genomic clone containing a bovine DR.beta.-like gene, BoDR.beta.-II, was isolated from a bovine genomic library and characterized by restriction enzyme mapping and nucleotide sequencing of exon regions. Alignment of this sequence with the human DR.beta. cDNA sequence allowed identification of exon/intron boundaries. The clone contains a 13.3 kilobase (kb) insert, and includes 1.3 kb 5'' of the .beta.1 exon and 6.7 kg 3'' of the transmembrane (TM) exon. Open reading frames were present in the BoDR.beta. exons sequenced. Nucleotide identities of the bovine .beta.1, .beta.2 and TM exons with the corresponding human DR.beta. exons were 73, 91 and 83%, respectively. Nucleotide identities of these exons with those of a previously described bovine DR.beta.-like pseudogene, BoDR.beta. I, were 69, 95 and 81%, respectively. Although a limited amount of sequence data was obtained for the intron regions, a 71% identity was found within a 514-nucleotide region immediately 3'' to the .beta.2 exons in BoDR.beta. I and BoDR.beta. II. A series of GT residues followed by a longer series of GA residues began about 35 nucleotides 3'' of the .beta.1 exon in both BoDR.beta. I and BoDR.beta. II.