Central dogma at the single-molecule level in living cells
Top Cited Papers
Open Access
- 20 July 2011
- journal article
- review article
- Published by Springer Nature in Nature
- Vol. 475 (7356) , 308-315
- https://doi.org/10.1038/nature10315
Abstract
Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time. This leads to heterogeneity in the messenger-RNA and protein copy numbers in a population of cells with identical genomes. With advanced single-cell fluorescence microscopy, it is now possible to quantify transcriptomes and proteomes with single-molecule sensitivity. Dynamic processes such as transcription-factor binding, transcription and translation can be monitored in real time, providing quantitative descriptions of the central dogma of molecular biology and the demonstration that a stochastic single-molecule event can determine the phenotype of a cell.This publication has 96 references indexed in Scilit:
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