NUTRITIONAL AND METABOLIC STUDIES OF DEBARYOMYCES HANSENII

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Abstract
The growth of strain NRRL Y-1448 of Debaryomyces hansenii was studied by using various growth media. A sodium chloride concentration of 2% to 3% and a pH of 5.5 were optimal for growth. No growth occurred below pH 4 and above pH 8. Either thiamine or biotin was required for normal growth. Growth occurred in media containing asparagine plus sodium acetate or asparagine plus citric acid but not citric acid plus acetate. Urea or ammonium sulphate were satisfactory nitrogen sources. The logarithmic phase of growth was reached twice as fast in media containing fructose as in media containing glucose. The metabolic products of growth were alcohol, acetic acid, pyruvic acid, and both free and esterified sterols.