OBSERVATIONS ON THE INTRAVAGINAL ASSAY OF NATURAL OESTROGENS USING AQUEOUS EGG ALBUMIN AS THE VEHICLE OF ADMINISTRATION

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Abstract
1. Oestrone dissolved in 1 % aqueous albumin, and administered by the intravaginal route, has 2·5–4·9 times the activity shown in 50% aqueous glycerol. 1 % aqueous egg albumin alone does not produce cornification of the vagina in the mouse. 2. When administered in 1 % aqueous egg albumin the relative potency of oestriol in terms of oestrone is 1·09, limits of error (P = 0·05) 0·70–1·70; and that of oestradiol-3:17β is 1·75, limits of error (P = 0·05) 1·15–2·66. 3. The slopes of the dose-response lines obtained with albumin are lower than the slopes obtained with glycerol but tests show the difference to be not quite significant at the 5 % level. There is no doubt, however, that the intravaginal route in general gives a lower slope than the subcutaneous route. 4. The slopes of the dose-response lines obtained in intravaginal tests using oestrone, oestriol and oestradiol-3:17β do not differ significantly. 5. The observed differences in slope have been discussed from the point of view of oestrogen action.

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