Enzymatic Dechorionation of Goldfish, Walleye, and Northern Pike Eggs
- 1 September 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 117 (5) , 456-460
- https://doi.org/10.1577/1548-8659(1988)117<0456:edogwa>2.3.co;2
Abstract
Removal ofthe chorion from fish eggs facilitates observation ofpiscine embryogenesis and allows efficient microinjection during gene transfer protocols. Several treatments were screened to evaluate their effectiveness in removing the chorion from newly fertilized eggs of goldfish Carassius auratus, walleye Stizostedion vitreum, and northern pike Esox lucius without destroying egg viability. Goldfish eggs dechorionated in 2.5 mg/mL trypsin showed excellent viability. No effective procedure for dechorionation of walleye eggs was found. Northern pike eggs could be dechorionated in 0.6 mg/mL protease type XXV, but mortalities above 50% were commonly observed.This publication has 2 references indexed in Scilit:
- Hatching in the teleostOryzias latipes: Limited proteolysis causes egg envelope swellingJournal of Experimental Zoology, 1983
- Proteolytic dechorionation of annual fish embryosThe Anatomical Record, 1977