ESTABLISHMENT OF CULTURED CELL LINES DERIVED FROM A HUMAN GASTRIC CARCINOMA

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 48  (1) , 61-68
Abstract
A human gastric carcinoma cell line, KATO-II, and its subline, KATO-III, were established in vitro from a pleural effusion of a 55-yr-old male patient. KATO-II cells were maintained using a culture medium containing human cord serum, and KATO-III cells were cultured with a medium containing fetal bovine serum. Both cell lines are morphologically similar; they grow in vitro floating free and have cytological features of signet ring cells. Population doubling times for KATO-II and KATO-III were 74 and 36 h, respectively. The modal chromosomal numbers of these cell lines fell in a tetraploid range. Heterotransplantation of KATO-II and KATO-III cells was successfully done in the cheek pouches of antithymocyte serum-treated hamsters. Microscopic appearance of the resultant tumors was consistent with poorly-differentiated adenocarcinoma comparable to the original tumor.

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