Plasmodesmata: a freeze-fracture view

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Abstract
The structure of plasmodesmata in the longitudinal walls of cells close to the meristematic region of Phaseolus vulgaris root tips were studied in freeze-fractured material not treated with glycerol. There were accumulations of membrane-associated particles on plasmalemmata at the junctions between plasmodesmata and plasmalemma. There was some indication that these particle clusters might be associated with the control of symplastic flow via sphincters into plasmodesmata. The junction between endoplasmic reticulum and the desmotubule, where visualized, appeared to be blocked. In other respects plasmodesmatal ultrastructure corresponded well with that described using conventional electron microscopical techniques.

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