Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects
- 23 April 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 5 (4) , 1219-1230
- https://doi.org/10.1021/bm034461z
Abstract
Reactions at ionizable functional groups in extracellular polymeric substances (EPS) from Bacillus subtilis are found to affect aqueous phase conformation and adsorption to mineral surfaces. Characterization by HPSEC, XPS, and FTIR indicates a wide range in apparent molecular mass (0.57−128 kDa), with functional group composition depending on cell growth phase (exponential vs stationary) and location in suspension (free vs cell-bound). ATR-FTIR spectroscopy shows complexation and dissociation of protons on acidic functional groups that result in α-helical protein conformation at pH < 2.6 and random coil (unordered) conformation at higher pH (>6). EPS exhibit higher affinity for adsorption to α-FeOOH than amorphous SiO2 because of surface charge effects. Increased amide II band intensity and an amide I band shift to higher frequency indicate changes in protein structure upon adsorption. Goethite-EPS spectra show emergent vibrations consistent with P−O−Fe bonding, which suggests a role of phosphodiester groups in the adsorption reaction.Keywords
This publication has 55 references indexed in Scilit:
- Characterization and Implications of the Cell Surface Reactivity of Calothrix sp. Strain KC97Applied and Environmental Microbiology, 2002
- Contributions of Bacterial Surface Polymers, Electrostatics, and Cell Elasticity to the Shape of AFM Force CurvesLangmuir, 2002
- Development of a Hierarchical Classification System with Artificial Neural Networks and FT-IR Spectra for the Identification of BacteriaApplied Spectroscopy, 2000
- Probing Bacterial Electrosteric Interactions Using Atomic Force MicroscopyEnvironmental Science & Technology, 2000
- An ATR−FTIR Study of Glyphosate and Its Fe(III) Complex in Aqueous SolutionEnvironmental Science & Technology, 1998
- Conformational Effects of Calcium Release from Parvalbumin: Comparison of Computational Simulations with Spectroscopic InvestigationsBiochemistry, 1997
- Identification of some bacterial cell components by FT-IR spectroscopyFEMS Microbiology Letters, 1995
- Infrared studies of interaction between metal ions and Ca2+‐binding proteins Marker bands for identifying the types of coordination of the side‐chain COO− groups to metal ions in pike parvalbumin (pI = 4.10)FEBS Letters, 1994
- Polymorphic phase behaviour of phospholipid membranes studied by infrared spectroscopyBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1984
- The structure of disodium deoxyguanosine-5'-phosphate tetrahydrateActa Crystallographica Section B: Structural Science, Crystal Engineering and Materials, 1974