Comparative modelling of major house dust mite allergen Der p I: structure validation using an extended environmental amino acid propensity table
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 7 (7) , 869-894
- https://doi.org/10.1093/protein/7.7.869
Abstract
Cysteine mutagenesis for the purpose of chemical labelling was applied to the K+ channel neurotoxin charybdotoxin, a 37-residue peptide with six functionally essential cysteines. An additional ‘spinster cysteine’ was introduced at a position far away in space from the toxin's known interaction surface where it contacts its K+ channel receptor. Despite the presence of the extra unpaired cysteine residue,the toxin still folds efficiently and may be labelled by fluorescent and radioactive reagents to give a functionally competent toxin.Keywords
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