The relationship between urinary prostaglandin excretion rates and urine flow in conscious rats. Evaluation of the radioimmunoassay by gas chromatography‐mass spectrometry

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Abstract
In conscious rats prostaglandin E2 and F2.alpha. excretion rates increased from about 20 to more than 100 pg/min per g kidney weight as urine flow decreased from about 10 to below 1 .mu.l/min per g kidney weight following 24 h of water deprivation, confirming previous data from surgically prepared rats (Leyssac and Christensen, 1981 b). There was no difference in this response between rats on a normal Na diet and severly Na-depleted rats. Additional supply of polyunsaturated fatty acids in the food did not influence the results. Elevated levels of prostaglandin excretion did not correlate with urine osmolality. The reliability of the radioimmunoassays used was documented by comparison with measurements by gas chromatography-mass spectrometry. These 2 methods provide almost identical estimates of urinary PGE2 and PGF2.alpha. content. Renal prostaglandin production is activated by a negative feedback mechanism as tubular and/or urine flow rate decreases toward subnormal values.

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