Differentiation of TRIC and LGV Organisms Based on Enhancement of Infectivity by DEAE-Dextran in Cell Culture
- 1 March 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 125 (3) , 313-317
- https://doi.org/10.1093/infdis/125.3.313
Abstract
Pretreatment of HeLa-229 cell monolayers by washing with DEAE-dextran (30 μg/ml) before inoculation of the trachoma-inclusion conjunctivitis (TRIC) agent resulted in enhanced infectivity, as shown by a 10-fold or greater increase in the number of inclusions and a two- to three-log greater infectivity. The infectivity for cell cultures of the morphologically and immunologically closely related lymphogranuloma venereum (LGV) organisms was not enhanced by treatment with DEAE-dextran. This effect provides a basis for initial classification of Chlamydia isolated from the human genital tract.Keywords
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