Cytochemistry of mouse mast cell reaction to polylysine

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Abstract
Summary The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 μg/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.