The Use of Deuterium Oxide as a Mobile Phase for Structural Elucidation by HPLC/UV/ESI/MS
- 14 September 2000
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 72 (20) , 5070-5078
- https://doi.org/10.1021/ac000316p
Abstract
The use of deuterium oxide as a mobile phase in the routine analysis of pharmaceutical compounds was investigated. The deuterium exchange of labile hydrogen atoms aids in structural confirmation and elucidation of unknown impurities and degradation products. Although deuterium oxide as a mobile phase does in some cases change the retention times, the changes in retention times do not interfere with the analysis. A study of the high-performance liquid chromatography system shows that equilibration times for the deuterium-containing mobile phases are similar to equilibration times with changes of other mobile phases. The use of this technique in the analysis of pharmaceutical compounds and other small molecules is presented.Keywords
This publication has 16 references indexed in Scilit:
- Conformational Effects of Substituting Methionine with (2S,3S)-2,3-Methanomethionine in Phe-Met-Arg-Phe-NH2Journal of the American Chemical Society, 1995
- Detection of Transient Protein Folding Populations by Mass SpectrometryScience, 1993
- Convergent evolution of similar enzymatic function on different protein folds: The hexokinase, ribokinase, and galactokinase families of sugar kinasesProtein Science, 1993
- Labile hydrogen counting in biomolecules using deuterated reagents in desorption chemical ionization and fast atom bombardment mass spectrometryAnalytical Chemistry, 1992
- Thermospray liquid chromatography/mass spectrometry in deuterium oxideAnalytical Chemistry, 1988
- Characterization of organic dyes aided by hydrogen-deuterium exchange in liquid secondary ion mass spectrometry (liquid SIMS)International Journal of Mass Spectrometry and Ion Processes, 1987
- Mass spectral characterization of nitrogen-containing compounds with ammonia chemical ionizationAnalytical Chemistry, 1982
- Catalysts for ChemistsAnalytical Chemistry, 1982
- Continuous Monitoring of Total Micro LC Eluant by Direct Liquid Introduction LC/MSJournal of Chromatographic Science, 1981
- Determination of active hydrogen in organic compounds by chemical ionization mass spectrometryAnalytical Chemistry, 1972