Nickel Grids Cause Astigmatism in the Transmission Electron Microscope
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 58 (6) , 373-374
- https://doi.org/10.3109/10520298309066815
Abstract
Nickel grids are used in various methods (e.g., in immunocytochemistry) where chemically inert grids are required. Recently (Neiss 1983) we have used formvar-coated nickel grids when removing osmium from mounted ultrathin sections with 10% periodic acid (Lewis and Knight 1977). This is possible because nickel, unlike copper, adequately withstands the oxidation necessary for osmium removal. Handling sections on nickel grids avoids the disadvantages of free-floating sections, whose use for osmium removal has been recommended by Lewis and Knight (1977, chapter 2.2.3).Keywords
This publication has 1 reference indexed in Scilit:
- The electron density of light and dark lysosomes in the proximal convoluted tubule of the rat kidneyHistochemistry and Cell Biology, 1983